The garden identified as Gethsemane is located at the foot of the Mount of Olives, now within the city of Jerusalem. Located by the garden is the Church of All Nations, also known as the Church of the Agony.

The ancient church was destroyed by the Sassanids in 614. The church rebuilt on the site by the Crusaders was finally razed, probably in 1219.

The name Gethsemane is given in the Greek of the Gospels (Matthew 26:36 and Mark 14:32) as Γεθσημανι (Gethsêmani). This represents the Aramaic (see also: Aramaic of Jesus) 'Gath-Šmânê', meaning 'the oil press' or 'oil vat' (referring to olive oil). It would appear from this that there were a number of olive trees planted around the area at the time.

The Garden of Gethsemane was a focal site for early Christian pilgrims. It was visited in 333 by the anonymous "Pilgrim of Bordeaux", whose Itinerarium Burdigalense is the earliest description left by a Christian traveler in the Holy Land. In his Onomasticon, Eusebius of Caesarea notes the site of Gethsemane "at the foot of the Mount of Olives", and he adds that "the faithful were accustomed to go there to pray".